√资源天堂中文在线丨黄色一级网丨少妇饥渴xxhd天美xxhd丨99精产国丨欧美成人精品视频在线不卡丨狠狠综合久久久久综合网站丨最近免费中文字幕中文高清百度丨中文在线www天堂网丨免费无码黄动漫在线观看丨99这里有精品视频视频丨久久久99国产精品免费丨黄色大全在线观看丨5678少妇影院丨成人性生交视频免费观看丨国产无套粉嫩白浆内谢在a丨久久国产主播福利在线丨欧洲精品久久久av无码电影丨亚洲国产良家在线观看丨欧美人与物videos另类丨人妻少妇88久久中文字幕丨日本真人做爰免费视频120秒丨国内精品九九久久精品丨av一卡二卡丨欧美视频网址丨成 人 黄 色 片 在线播放

【文獻標(biāo)題】Phosphate regulates malate/citrate-mediated iron uptake and transport in apple

【作者】Jiu-Cheng Zhang，Xiao-Na Wang，Wei Sun，et.al

【作者單位】山東農(nóng)業(yè)大學(xué)（Shandong Agricultural University）

【文獻中引用產(chǎn)品】

植物蘋果酸(MALIC ACID)ELISA試劑盒

植物檸檬酸（Citric Acid）ELISA試劑盒

【關(guān)鍵詞】Low phosphate，Iron-deficiency，Absorption，Organic acids，Apple

【DOI】doi.org/10.1016/j.plantsci.2020.110526

【影響因子(IF)】5.90

【出版期刊】《Plant Science》

【產(chǎn)品原文引用】

Rhizosphere organic acid efflux collection and measurement

The apple plants were treated in hydroponic nutrient solution for 4 days (pH = 5.8), and the nutrient solution was then dried to a powder in a -80℃ freeze dryer. The powder was dissolved with 5 ml of deionized water, the solution was then passed through a cation exchange resin and the filtrate was collected. After lyophilizing again, the sample was dissolved in 5 ml of deionized water and stored at -20℃. The organic acid efflux was analyzed via enzyme-linked immunosorbent assay (ELISA; Hengyuan, Shanghai, China). Organic acid was made into a solid-phase antibody, and horseradish peroxidase (HRP) was then combined with the organic acid antibody to form an antibody-antigenenzyme-labeled antibody complex. After thorough washing, the TMB substrate was added to develop the color, with the TMB converted to blue under the catalysis of HRP and finally converted to yellow under the action of acid. The shade of the color positively correlated with the organic acid concentration in the sample. Finally, the absorbance was measured using a microplate reader at a wavelength of 450 nm. The organic acid concentration was calculated from a standard curve.Effluent malate content = (337.57 ×A450 –9.0216) ×5 ×V / (134.09× M). Effluent citrate content = (506.82 ×A450 –17.148) ×5 ×V /(192.13 × M). (A: absorbance, V: volume of filtrate, M: fresh weight of apple root).

完整版PDF文獻請咨詢在線客服或者聯(lián)系我司業(yè)務(wù)員！

更多公司福利請關(guān)注“恒遠生物”VX公眾號！